Of course, these can be altered to fit your gene of interest. I usually select ‘ mRNA’ as the ‘ Molecular types’ and the ‘ Homo sapiens’ as the ‘ Organism’. On the results pages, you can also use the sidebars to filter the search further. Since the NCBI database is vast, you will want to narrow down your search the best you can.Īs an example, I am interested in the interleukin-6 gene in humans. I usually search my full gene name along with the species I am interested in. Search for your gene of interest using the search bar at the top. Specifically, the part of NCBI we are interested in is their Nucleotide section. So, you need to head on over to the NCBI website. Mastering qPCRįurther video tutorials on qPCR primer design can be found in our Mastering qPCR course. ![]() ![]() Here is how to design real-time PCR primers using Primer-BLAST. Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers will bind to unspecific regions of the organism’s genome via the BLAST algorithm.
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